One of the hallmarks of many DNA polymerases is that they proofread as they copy DNA, ensuring high-fidelity duplicates of an organism’s genome. This error-checking ability is missing from enzymes ...

Surface model of SpCas9–reverse transcriptase–pegRNA–target DNA complex. The prime editor, composed of a SpCas9 and a reverse transcriptase, reverse transcribes template sequence in pegRNA, resulting ...

Joint research led by Yutaro Shuto, Ryoya Nakagawa, and Osamu Nureki of the University of Tokyo determined the spatial structure of various processes of a novel gene-editing tool called “prime editor.

A newly revealed drug-binding site on human immunodeficiency virus reverse transcriptase could lead to a new class of HIV medications. Structures of drug complexes of HIV reverse transcriptase have ...

With two catalytic activities and many substrates, how does HIV's reverse transcriptase enzyme know what to do to which substrate? Zooming in on the enzyme's molecular interactions provides ...

The creation of cDNA from RNA templates is an integral part of molecular biology that is mediated by reverse transcriptase (RT) enzymes. Unfortunately, these proteins ...

With more than 20 drugs currently licensed to treat HIV infection 1 and over 200 mutations associated with drug resistance 2,3,4,5, it is increasingly difficult to develop a comprehensive ...

Just like us, bacteria are under constant threat of infection by viruses (called phages). We’ve found that many bacteria are armed with sophisticated viral defence systems powered by reverse ...